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Standard package for QIAsymphony and Rotor-Gene Q connection, including QIAlink software license, assessment of laboratory infrastructure, alignment with LIMS provider for implementation of QIAlink interface in LIMS, on-site installation, configuration, post-installation testing, and user training.Įasy routine verification Optical temperature verification (OTV) is a method that verifies the in-tube temperature in a Rotor-Gene Q MDx. After assay setup, the assays are then transferred to the Rotor-Gene Q MDx for PCR and data analysis.

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Nucleic acids are purified using the designated manual or automated purification kit that is validated for the downstream assay. Convenient features of the Rotor-Gene Q MDx include: This saves time and costs, and keeps the instrument operational for continuous testing. Minimum maintenance maximum convenience The Rotor-Gene Q MDx is engineered to reduce the need for maintenance, and to maximize ease of use. Channels for optical detection Channel Excitation (nm) Detection (nm) Green 470 ± 10 510 ± 5 Yellow 530 ± 5 557 ± 5 Orange 585 ± 5 610 ± 5 Red* 625 ± 5 660 ± 10 Crimson 680 ± 5 712 high pass HRM* 460 ± 20 510 ± 5 * The red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests. The fixed optical path ensures consistent excitation for every sample, which means that there is no need to use a passive internal reference dye such as ROX. Energy is transmitted through the thin walls at the base of the tube.Įmitted fluorescence passes through emission filters on the side of the chamber and is then collected by a photomultiplier. Samples are excited from the bottom of the chamber by a light-emitting diode (see figure '). Robust optics for multiplex applications With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis (see figure ').

Unique rotary design for outstanding performance The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction conditions. With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis. It is highly suited for in vitro diagnostic applications. The Rotor-Gene Q MDx is an instrument that enables real-time PCR. Tubes spin past the excitation/detection optics every 150 milliseconds enabling high-speed data capture. Heating/cooling is achieved by rapid airflow in the reaction chamber.